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2.3 Performmagneticseparation. Transfer the supernatant into anewtube for further analysis, if desired. The supernatant is the non-binding fraction. 2.4 Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into amagneticstand to collect the beads against the side of the tube.

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The biotinylated molecules (e.g. peptides, proteins, antibodies, sugars, lectins, oligonucleotides, DNA/RNA) bind to the beads due to thehighaffinity between streptavidin and biotin. MCE StreptavidinMagneticBeads are removed from the solution manually by using amagneticstand or automatically by using an instrument.

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